Stable, recombinant AequoScreen® cell line expressing aequorin and the Muscarinic acetylcholine M5 receptor, human recombinant in CHO-K1 host cells.
Revvity's AequoScreen® double transfected cell lines are provided in two vials of frozen cells, each containing ∽2.5 x 106 cells. Detailed product information as well as recommended cell culture conditions and validation assay date are available each cell line.
AequoScreen technology is a generic GPCR technology that can be used with Gs, Gi, and Gq-coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of resulting flash luminescence signal.
Features:
Revvity's AequoScreen cell lines require accepted cell line Terms and Conditions prior to order processing. Additional agreement needed commercial use. Contact us using Request More Information button above additional information regarding required documentation.
Assay Validation |
Binding
Calcium Fluorescence
Calcium Luminescence
|
---|---|
Assay Target Class |
GPCR
|
Assay Target Type |
Cell line
|
Brand |
AequoScreen
|
G-Alpha Natural Receptor |
Gq/G11
|
Host |
CHO-K1
|
Receptor Type |
Acetylcholine (Muscarinic)
Muscarinic
|
Second Messenger Release |
Calcium flux
|
Species |
Human
|
Shipping Conditions |
Shipped in Dry Ice
|
Special Ordering Information |
Requires accepted Terms and Conditions. Additional agreement needed for commercial use.
|
Therapeutic Area |
Cardiovascular
Gastrointestinal
Parasympathetic Nervous System
|
Unit Size |
5 million cells
|