PerkinElmer

Kinase

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Study phosphorylation of protein, peptide, and lipid substrates for kinase inhibition, screening, and mechanism-of-action studies. We offer a range of end-point and real-time kinetic assay kits, technologies, and instruments such as:

  • Labeled substrates for kinetic assays and antibody-free screening
  • Alpha kits for highly-sensitive detection of cellular phosphorylation
  • Fast and simple TR-FRET assays using pre-validated peptide and protein substrates derived from biologically-relevant targets
  • Microfluidic mobility-shift assays (MSA) for ratiometric analysis of substrate conversion
  • Multi-mode plate readers for maximal speed and throughput independent of technology platform

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Antibody Conjugates
  • Anti-P-Ser 14-3-3 (1)
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Assay points(200-50,000)
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  • 4EBP1 (3)
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  • STAT5 (3)
  • STAT6 (1)
  • SYK (2)
  • Topo IIα (2)
  • VEGF2 Receptor (2)
  • β-Catenin (1)
Assay Target Class
  • Phosphoprotein (126)
  • Protein (21)
Automation Compatible
  • Yes (203)
Buffer
  • Aqueous (1)
  • Tricine (7)
  • Tricine with stabilizer and dye (6)
Concentration
  • 10 mCi/mL (8)
  • 12.5 mCi/mL (3)
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Detection Method
  • Absorbance (1)
  • Alpha (133)
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  • Radiometric (16)
  • Time-Resolved Fluorescence (TRF) (67)
  • TR-FRET (61)
Experimental Type
  • In vitro (158)
Fluorophore
  • Eu-N1 (5)
  • Eu-W1024 (22)
  • SureLight-APC (1)
  • ULight (19)
Format
  • Microplates (173)
Label Position
  • alpha (1)
  • gamma (15)
Maximum Temperature (Celsius)
  • 40-50 (2)
  • 50-60 (2)
Molecular Modification
  • Phosphorylation (118)
Nucleotide
  • ATP (12)
  • GTP (4)
Product Brand Name
  • Alpha SureFire Ultra Multiplex (8)
  • AlphaLISA SureFire Ultra (51)
  • AlphaPlex (8)
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  • LabChip EZ Reader (44)
  • LANCE (4)
  • LANCE Ultra (16)
  • LANCE SureLight (1)
  • LANCE Ultra (37)
  • NEN (2)
Radionuclide
  • 32P (9)
  • 33P (2)
  • 35S (5)
Radioisotope
  • P-32 (9)
  • P-33 (2)
  • S-35 (5)
Sample Type
  • Enzyme (35)
Target Species
  • Human (126)

"Kinase" 1-50 of 268 Products and Services

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EnSight multimode plate-reader

EnSight Multimode Plate Reader

The EnSight multimode plate reader combines labeled, label-free* & image-based cytometry technologies in a single benchtop platform. Imaging & online data analysis is fast: a 384-well plate can be imaged in less than 5 mins. The system’s modular design, combined with workflow-based Kaleido data acquisition & analysis software, gives a truly versatile plate reader that gets users productive quickly.

*Label free detection module of EnSight Plate Reader is available outside the EU. Please contact your local representative for further details.

PerkinElmer

LANCE Ultra Europium-anti-phospho-Histone H3 (Thr3) Antibody

Rabbit monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from Histone H3 phosphorylated at Thr3. Histone H3 peptides can be phosphorylated by several kinases including RSK2. Sequence motif recognized: ARTKQTARKSTGGKAPRKQLAGCG
Price Range : 1800.00 CAD - 8400.00 CAD

AlphaScreen Phosphotyrosine (PY20) Assay Kit

AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine PY20 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
Price Range : 215.00 CAD - 16300.00 CAD
PerkinElmer

LANCE Ultra Europium-anti-phospho-Acetyl-CoA Carboxylase (Ser79) Antibody

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from rat Acetyl-CoA Carboxylase phosphorylated at Ser79. Acetyl-CoA Carboxylase peptides can be phosphorylated by the AMPKα1 kinase. Sequence motif recognized: SSM-pS-GL
Price Range : 2400.00 CAD - 8400.00 CAD

GTP, [γ-32P]- 6000Ci/mmol 10mCi/ml , 250 µCi

Guanosine triphosphate (GTP), labeled on the gamma phosphate with 32P, is used as a substrate by some kinases in phosphorylation assays.
List Price : 740.00 CAD

AlphaScreen PhosphoSensor Kit

Antibody-free assay for kinase activity via dectection of phosphorylated protein or peptide substrate.
Price Range : 430.00 CAD - 3400.00 CAD
SureFire principle

AlphaScreen SureFire cJun (p-Ser63) Assay Kit

The AlphaScreen® SureFire® p-cJun (Ser63) assay is a sandwich immunoassay for quantitative detection of phospho-cJun (phosphorylated on Ser63) in cellular lysates using Alpha Technology.
Price Range : 1750.00 CAD - 19100.00 CAD
PerkinElmer

LANCE Ultra Europium-anti-phospho-CREB (Ser133)

Monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Ser133 in the cAMP response element binding (CREB) protein. CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.
Price Range : 1300.00 CAD - 5700.00 CAD
PerkinElmer

LANCE Ultra Europium-anti-phospho-PKC (Ala25Ser) peptide Antibody

Mouse monoclonal antibody labeled with the LANCE® Europium chelate. This antibody detects phospho-Ser in a synthetic peptide (RFARKGSLRQKNV) only after the peptide is phosphorylated by PKC or PKA kinases. The synthetic peptide is derived from amino acids 19-31 of human PKCα in which Ala25 is mutated to Ser. Sequence motif recognized: RFARKG-pS-LRQKNV
Price Range : 2400.00 CAD - 8400.00 CAD

LANCE Ultra ULight™-poly GT

Poly Glu-Tyr (4:1) labeled with ULightTM dye. This random polymer is a Tyrosine kinase substrate.
Price Range : 380.00 CAD - 1000.00 CAD
PerkinElmer

ProfilerPro Kinase Peptide Substrate 29, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 29 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 720.00 CAD

AlphaScreen Phosphotyrosine (PT66) Assay Kit

AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine PT66 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
Price Range : 215.00 CAD - 16300.00 CAD
SureFire principle

AlphaScreen SureFire Phospho-Caspase 9 (Ser196) Kit

The AlphaScreen® SureFire® p-Caspase 9 (Ser196) assay is a sandwich immunoassay for quantitative detection of phospho-Caspase 9 (phosphorylated on Ser196) in cellular lysates using Alpha Technology
Price Range : 1750.00 CAD - 19100.00 CAD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra AKT 1/2/3 (pS473) Assay Kit

The AlphaLISA® SureFire® Ultra p-AKT1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.
Price Range : 580.00 CAD - 33600.00 CAD
PerkinElmer

LANCE Ultra Europium-anti-phospho-40S Ribosomal Protein S6 (Ser235/236)

Europium-labeled rabbit monoclonal antibody recognizing human 40S ribosomal protein S6 phosphorylated at both Ser235 and Ser236. Sequence motif recognized: RRL(pS)(pS)LRA
Price Range : 850.00 CAD - 2950.00 CAD
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-SMAD1 (Ser463/465) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-SMAD1 (Ser463/465) kits are designed for the detection of SMAD1 (phosphorylated at S463/465) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1750.00 CAD - 7900.00 CAD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-ERK1/2 (Thr202/Tyr204) Assay Kit

The AlphaLISA® SureFire® Ultra p-ERK 1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK 1/2 (phosphorylated on Thr202/Tyr204 in ERK1, or Thr185/Tyr187 in ERK2) in cellular lysates using Alpha Technology.
Price Range : 580.00 CAD - 33600.00 CAD
PerkinElmer

LANCE Ultra Europium-anti-phospho-Histone H3 (Ser10) Antibody

Mouse monoclonal antibody labeled with the LANCE Europium W-1024-ITC chelate. This antibody detects peptides derived from Histone H3 phosphorylated by Ser10. Histone H3 peptides can be phosphorylated by several kinases, including Aurora B. Sequence motif recognized: ARTKQTARKSTGGKAPRKQLAGCG
Price Range : 2400.00 CAD - 8400.00 CAD

ATP, [γ-33P]- 3000Ci/mmol, 10mCi/ml

Adenosine triphosphate, labeled on the gamma phosphate group with 33P.
Price Range : 510.00 CAD - 1400.00 CAD

LANCE Ultra ULight™-phospho-40S ribosomal protein S6 (pSer235/236) Peptide

Synthetic peptide containing residues surrounding Ser235 and Ser236 of human 40S ribosomal protein S6; this peptide is pre-phosphorylated on Ser235; phosphorylation site: Ser236. This peptide is phosphorylated at Ser236 efficiently by kinases from the RSK family. Phosphorylation motif: RRL(pS)SLRA
Price Range : 460.00 CAD - 1000.00 CAD
SureFire principle

AlphaScreen SureFire Phospho-c-Met (Tyr1234/1235) Assay Kit 

The AlphaScreen® SureFire® p-c-Met (Tyr1234/1235) assay is a sandwich immunoassay for quantitative detection of phospho-c-Met (phosphorylated on Tyr1234/1235) in cellular lysates using Alpha Technology.
Price Range : 1750.00 CAD - 19100.00 CAD

LANCE Ultra ULight-TK peptide

ULight™-labeled synthetic peptide containing 28 amino acid peptide with 8 Tyrosine residues in different amino acid context that broadly provides for the recognition specificity of greater than 85% of 83 tested cytoplasmic and receptor tyrosine kinases.
Price Range : 460.00 CAD - 1350.00 CAD
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

DELFIA Eu-N1 Labeled Anti-Phosphothreonine Antibody, 10µg

Europium-labeled anti-phosphothreonine antibody, for use in DELFIA® time-resolved fluorescence (TRF) kinase assays.
List Price : 1500.00 CAD

LANCE Ultra ULight™-Crosstide

Crosstide peptide (sequence: CGSGSGRPRTSSFAEG) labeled with ULight™ dye. Synthetic peptide derived from glycogen synthase kinase-3 alpha (GSK-3α) (Phosphorylation site: Ser21). GSK-3α can be phosphorylated by a variety of kinases including AKT1, AKT2, AKT3, MSK1, AMS2, PDK1, PLK3.
Price Range : 380.00 CAD - 1000.00 CAD
SureFire principle

AlphaScreen SureFire CREB (p-Ser133) Assay Kit

The AlphaScreen® SureFire® p-CREB (Ser133) assay is a sandwich immunoassay for quantitative detection of phospho-CREB (phosphorylated on Ser133) in cellular lysates using Alpha Technology.
Price Range : 1950.00 CAD - 20900.00 CAD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) Assay Kit

The AlphaLISA® SureFire® Ultra p-SMAD1 (Ser463/Ser465) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD1 (phosphorylated on Ser463/Ser465) in cellular lysates using Alpha Technology.
Price Range : 580.00 CAD - 33600.00 CAD

AlphaScreen® P-Tyr-100 (Phosphotyrosine) Assay Kit

AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine P-Tyr-100 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
Price Range : 215.00 CAD - 16300.00 CAD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-eIF2α (Ser51) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-eIF2α (Ser51) assay is a sandwich immunoassay for quantitative detection of phospho-eIF2α (phosphorylated on Ser51) in cellular lysates using Alpha Technology.
Price Range : 580.00 CAD - 33600.00 CAD
SureFire principle

AlphaScreen SureFire Phospho-cofilin (Ser3) Assay Kit

The AlphaScreen® SureFire® p-Cofilin (Ser3) assay is a sandwich immunoassay for quantitative detection of phospho-Cofilin (phosphorylated on Ser3) in cellular lysates using Alpha Technology.
Price Range : 1750.00 CAD - 19100.00 CAD
SureFire principle

AlphaScreen SureFire JNK 1-3 (p-Thr183;Tyr185) Assay Kit

The AlphaScreen® SureFire® p-JNK1/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology.
Price Range : 1750.00 CAD - 19100.00 CAD
SureFire principle

AlphaScreen SureFire IkBalpha (p-Ser32/Ser36) Assay Kit

The AlphaScreen® SureFire® p-IκBα (Ser32/36) assay is a sandwich immunoassay for quantitative detection of phospho-IκBα (phosphorylated on Ser32/36) in cellular lysates using Alpha Technology.
Price Range : 1750.00 CAD - 19100.00 CAD

ATP, [γ-33P]- 3000Ci/mmol, 10mCi/ml

Adenosine triphosphate, labeled on the gamma phosphate group with 33P.
Price Range : 850.00 CAD - 2150.00 CAD
PerkinElmer

LANCE Ultra ULight™-Histone H3 (Thr3/Ser10) Peptide

ULight™-labeled synthetic peptide derived from residues 463-480 of human RAC-alpha serine/threonine-protein kinase (AKT1 or PKB). AKT/PKB can be phosphorylated at Ser473 by the mTOR/Rictor complex and MAPKAP kinase 2. Peptide sequence : CSERRPHFPQFSYSASGTAR
Price Range : 350.00 CAD - 1400.00 CAD

LANCE Ultra ULight™-IkappaB-alpha (Ser32/36) peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Ser32/36 of human IkappaB-alpha. The ULight-IkappaB-alpha peptide can be phosphorylated at Ser 32/36 by the IKKβ kinase. Peptide core motif: RHDSGLDSM
Price Range : 360.00 CAD - 990.00 CAD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-VEGF Receptor 2 (Tyr1175) Assay Kit

The AlphaLISA® SureFire® Ultrap-VEGF Receptor 2 (Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGFR Receptor 2 (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.
Price Range : 580.00 CAD - 33600.00 CAD
PerkinElmer

LANCE Eu-W1024 Anti-phosphotyrosine (PY20)

Europium-labeled anti-phosphotyrosine (PY20) antibody, for use in LANCE® TR-FRET kinase assays.
Price Range : 1250.00 CAD - 10200.00 CAD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total SYK Assay Kit

The AlphaLISA® SureFire® Ultra Total SYK assay is a sandwich immunoassay for quantitative detection of SYK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 580.00 CAD - 33600.00 CAD
PerkinElmer

LANCE Ultra Europium-anti-phospho-eIF4E-binding protein 1 (Thr37/46)

Europium-labeled rabbit monoclonal antibody recognizing human eukaryotic translation initiation factor 4E-binding protein 1 phosphorylated at Thr37 or Thr46. Sequence motif recognized: ST(pT)PGGTLFST(pT)PG
(mono-phosphorylated peptides at either residue will also be successfully recognized)
Price Range : 2400.00 CAD - 8400.00 CAD

LANCE Ultra ULight™-eIF4E-binding protein 1 (Thr37/46) Peptide

Synthetic peptide containing residues surrounding Thr37 and Thr46 of human eukaryotic translation initiation factor 4E-binding protein 1; phosphorylation sites: Thr37 and Thr46. The ULight-eIF4E-binding protein 1 peptide can be phosphorylated at Thr37/46 by mTOR and many kinases. Phosphorylation motif: STTPGGTLFSTTPG
Price Range : 460.00 CAD - 1000.00 CAD
alphascreen surefire total

AlphaScreen SureFire Monoclonal Total ERK, 50,000 Assay Points

The AlphaScreen® SureFire® total ERK assay is a sandwich immunoassay for quantitative detection of ERK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. This assay performs similarly to the TGRTES SureFire kit, but contains a monoclonal antibody for ERK detection.
List Price : 21000.00 CAD
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-ERK 1/2 (Thr202/Tyr204) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-ERK 1/2 (Thr202/Tyr204) kits are designed for the detection of phosphorylated ERK 1 and ERK2 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1750.00 CAD - 7900.00 CAD
SureFire principle

AlphaScreen SureFire Phospho-ATF2 (Thr71) Assay Kit

The AlphaScreen® SureFire® p-ATF2 (Thr71) assay is a sandwich immunoassay for quantitative detection of phospho-ATF2 (phosphorylated on Thr71) in cellular lysates using Alpha Technology.
Price Range : 1750.00 CAD - 19100.00 CAD
PerkinElmer

LANCE Ultra ULight™-Tyrosine Hydroxylase (Ser40) peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Ser40 of tyrosine hydoxylase. The ULight-tyrosine hydroxylase peptide can be phosphorylated at Ser40 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif : RRQSLIE
Price Range : 360.00 CAD - 990.00 CAD
SureFire principle

AlphaScreen SureFire p53 (p-Ser15) Assay Kit

The AlphaScreen® SureFire® p-p53 (p-Ser15) assay is a sandwich immunoassay for quantitative detection of phospho-p53 (phosphorylated on Ser15) in cellular lysates using Alpha Technology.
Price Range : 480.00 CAD - 19100.00 CAD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Ribosomal Protein S6 (Ser240/244) Assay Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-Ribosomal Protein S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha no-wash technology.
List Price : 1950.00 CAD
SureFire principle

AlphaScreen SureFire Phospho-GSK 3a (Ser21) Kit

The AlphaScreen® SureFire® p-GSK 3α (Ser21) assay is a sandwich immunoassay for quantitative detection of phospho-GSK3α (phosphorylated on Ser21) in cellular lysates using Alpha Technology.
Price Range : 1750.00 CAD - 19100.00 CAD
SureFire principle

AlphaScreen SureFire S6RP (p-Ser240;Ser244) Assay Kit

The AlphaScreen® SureFire® p-Ribosomal Protein S6 (p-Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-RP S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha Technology.
Price Range : 1750.00 CAD - 19100.00 CAD
PerkinElmer

ProfilerPro Kinase Peptide Substrate 22, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 22 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 720.00 CAD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-IGF-1 Receptor β (Tyr1135/1136) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-IGF-1 Receptor β (Tyr1135/1136) assay is a sandwich immunoassay for quantitative detection of phospho-IGF Receptor β (phosphorylated on Tyr1135/1136) in cellular lysates using Alpha Technology.
Price Range : 580.00 CAD - 33600.00 CAD
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-STAT3 (Tyr705) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-STAT3 (Tyr705) kits are designed for the detection of phosphorylated STAT3 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1750.00 CAD - 7900.00 CAD

"Kinase" 1-50 of 268 Products and Services

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