PerkinElmer

Kinase

OF

Study phosphorylation of protein, peptide, and lipid substrates for kinase inhibition, screening, and mechanism-of-action studies. We offer a range of end-point and real-time kinetic assay kits, technologies, and instruments such as:

  • Labeled substrates for kinetic assays and antibody-free screening
  • Alpha kits for highly-sensitive detection of cellular phosphorylation
  • Fast and simple TR-FRET assays using pre-validated peptide and protein substrates derived from biologically-relevant targets
  • Microfluidic mobility-shift assays (MSA) for ratiometric analysis of substrate conversion
  • Multi-mode plate readers for maximal speed and throughput independent of technology platform

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Antibody conjugates
  • Anti-P-PKA (1)
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  • Kinase (135)
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Assay Target
  • 4EBP1 (3)
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  • STAT5 (2)
  • Topo IIα (2)
  • VEGF2 Receptor (2)
  • β-Catenin (1)
Assay Target Class
  • Phosphoprotein (112)
  • Protein (10)
Automation Compatible
  • Yes (181)
Buffer
  • Aqueous (1)
  • Tricine (7)
  • Tricine with stabilizer and dye (6)
Concentration
  • 10 mCi/mL (8)
  • 12.5 mCi/mL (3)
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Detection Method
  • Absorbance (1)
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  • TR-FRET (44)
Experimental type
  • In vitro (161)
Fluorophore
  • 5-FAM (29)
  • Eu-N1 (6)
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  • SureLight-APC (1)
  • ULight (19)
Format
  • Microplates (176)
Label Position
  • alpha (1)
  • gamma (15)
Maximum Temperature (Celsius)
  • 40-50 (2)
  • 50-60 (2)
Molecular modification
  • Phosphorylation (121)
Nucleotide
  • ATP (12)
  • GTP (4)
Product Brand Name
  • Alpha SureFire Ultra Multiplex (8)
  • AlphaLISA SureFire Ultra (45)
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  • LANCE SureLight (1)
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  • NEN (2)
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Radionuclide
  • 32P (9)
  • 33P (2)
  • 35S (5)
Radioisotope
  • P-32 (9)
  • P-33 (2)
  • S-35 (5)
Sample Volume
  • 2 mL (1)
  • 35 mL (1)
  • 400 mL (1)
Target Species
  • Human (107)
Volume
  • 0-50 (72)
  • 51-100 (4)
  • 201-250 (16)
  • 351-400 (1)
  • 451-500 (46)
  • Over 500 (42)

"Kinase" 1-50 of 248 Products and Services

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EnSight multimode plate-reader

EnSight Multimode Plate Reader

The EnSight multimode plate reader combines labeled, label-free & image-based cytometry technologies in a single benchtop platform. Imaging & online data analysis is fast: a 384-well plate can be imaged in less than 5 mins. The system’s modular design, combined with workflow-based Kaleido data acquisition & analysis software, gives a truly versatile plate reader that gets users productive quickly.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-eIF4E (Ser209) Assay Kits

The AlphaLISA® SureFire® Ultra™ p-eIF4E(Ser209) assay is a sandwich immunoassay for quantitative detection of phospho-eIF4E (phosphorylated on Ser209) in cellular lysates using Alpha Technology.
Price Range : 580.00 CAD - 33600.00 CAD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-ERK1/2 (Thr202/Tyr204) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-ERK 1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK 1/2 (phosphorylated on Thr202/Tyr204 in ERK1, or Thr185/Tyr187 in ERK2) in cellular lysates using Alpha Technology.
Price Range : 580.00 CAD - 33600.00 CAD
PerkinElmer

PDE Substrate iFL-cGMP, for LabChip EZ Reader

Fluorescently-labelled cGMP for Phosphodiesterase Assays (Enyzme inhibiton, target discovery, lead optimization)
List Price : 720.00 CAD

DELFIA Eu-N1 labeled anti-phosphoserine antibody, 10µg

Europium-labeled anti-phosphoserine antibody, for capturing antibodies in DELFIA® TRF kinase assays.
List Price : 1500.00 CAD
PerkinElmer

ProfilerPro Kinase Peptide Substrate 13, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 13 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 720.00 CAD

DELFIA Eu-N1 Labeled Anti-Phosphothreonine Antibody, 10µg

Europium-labeled anti-phosphothreonine antibody, for use in DELFIA® time-resolved fluorescence (TRF) kinase assays.
List Price : 1500.00 CAD

AlphaScreen PhosphoSensor Kit

Antibody-free assay for kinase activity via dectection of phosphorylated protein or peptide substrate.
Price Range : 430.00 CAD - 3400.00 CAD
PerkinElmer

ProfilerPro Kinase Peptide Substrate 11, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 11 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 720.00 CAD

LANCE Ultra ULight™-CREBtide

CREBtide peptide (sequence: CKRREILSRRPSYRK) labeled with ULight™ dye. Synthetic peptide derived from human cAMP Response Element Binding (CREB) Protein (phosphorylation site: Ser133). CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.
Price Range : 280.00 CAD - 1000.00 CAD
SureFire principle

AlphaScreen SureFire Phospho-MEK 1 Kit

The AlphaScreen® SureFire® p-MEK1 (Ser218/222) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha Technology.
Price Range : 1750.00 CAD - 19100.00 CAD

DELFIA Eu-N1 Anti-Phosphtyrosine (P-Tyr-100)

Europium-labeled anti-phosphotyrosine (P-Tyr-100) antibody, for use in DELFIA® time-resolved fluorescence (TRF) kinase assays, phosphoprotein detection, and phosphoprotein binding assays.
Price Range : 290.00 CAD - 3850.00 CAD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra AKT 1/2/3 (pS473) Assay Kit

The AlphaLISA® SureFire® Ultra p-AKT1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.
Price Range : 580.00 CAD - 33600.00 CAD
PerkinElmer

LANCE Ultra ULight™-Tyrosine Hydroxylase (Ser40) peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Ser40 of tyrosine hydoxylase. The ULight-tyrosine hydroxylase peptide can be phosphorylated at Ser40 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif : RRQSLIE
Price Range : 360.00 CAD - 990.00 CAD
PerkinElmer

LANCE Ultra ULight-p70 S6K (Thr389) Peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Thr389 of human p70 S6K. The ULight-p70 S6K peptide can be phosphorylated at Thr389 by several kinases including mTOR, COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1, and TAOK2. Phosphorylation motif: LGFTYVAP
Price Range : 350.00 CAD - 1400.00 CAD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD3 (Ser423/425) Assay Kit

The AlphaLISA® SureFire® Ultra p-SMAD3 (Ser423/Ser425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/Ser425) in cellular lysates using Alpha Technology.
Price Range : 580.00 CAD - 33600.00 CAD
SureFire principle

AlphaScreen SureFire IKKa (p-Ser176/Ser180) Assay Kit

The AlphaScreen® SureFire® p-IKKα (Ser176/180) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser176/180) in cellular lysates using Alpha Technology.
Price Range : 1950.00 CAD - 21000.00 CAD
Alpha SureFire Ultra Multiplex kits

Alpha SureFire® Ultra™ HV Multiplex p-ERK 1/2 (Eu) + Total ERK 545 (Tb) Kit

The Alpha SureFire® Ultra™ HV Multiplex p-ERK 1/2 + Total ERK assay kit is used to measure both the phosphorylation (Thr202/Tyr204) and total levels of endogenous “extracellular signal-regulated kinase 1 and 2” (ERK 1/2) in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure ERK 1/2 activation by either recombinant or endogenous receptors, and can be applied to primary cells.
Price Range : 1050.00 CAD - 60600.00 CAD

LANCE® Eu-W1024 phospho-(Ser) 14-3-3 binding motif antibody, 10 µg

Europium-labeled anti-phospho-(Ser) 14-3-3 motif antibody, for use in LANCE® TR-FRET kinase assays.
List Price : 4050.00 CAD

LANCE Ultra ULight™-IkappaB-alpha (Ser32/36) peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Ser32/36 of human IkappaB-alpha. The ULight-IkappaB-alpha peptide can be phosphorylated at Ser 32/36 by the IKKβ kinase. Peptide core motif: RHDSGLDSM
Price Range : 360.00 CAD - 990.00 CAD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-CREB (Ser133) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-CREB (Ser133) assay is a sandwich immunoassay for quantitative detection of phospho-CREB (phosphorylated on Ser133) in cellular lysates using Alpha Technology.
Price Range : 580.00 CAD - 33600.00 CAD
PerkinElmer

LANCE Ultra Europium-anti-phospho-Myelin Basic Protein (MBP)

Mouse monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Thr232 in myelin basic protein (MBP; Swiss-Prot: P02686). MBP can be phosphorylated by a variety of kinases including Erk1/Erk2. Sequence motif recognized: FFKNIVTPRpTPPPSQGK
Price Range : 350.00 CAD - 7100.00 CAD
SureFire principle

AlphaScreen SureFire JNK 1-3 (p-Thr183;Tyr185) Assay Kit

The AlphaScreen® SureFire® p-JNK1/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology.
Price Range : 1750.00 CAD - 19100.00 CAD
SureFire principle

AlphaScreen SureFire Phospho-beta catenin (Ser45) Assay Kit

The AlphaScreen® SureFire® p-beta catenin (Ser45) assay is a sandwich immunoassay for quantitative detection of phospho-beta catenin (phosphorylated on Ser45) in cellular lysates using Alpha Technology.
Price Range : 1750.00 CAD - 19100.00 CAD

LANCE Ultra ULight™-poly GT

Poly Glu-Tyr (4:1) labeled with ULightTM dye. This random polymer is a Tyrosine kinase substrate.
Price Range : 380.00 CAD - 1000.00 CAD
SureFire principle

AlphaScreen SureFire Phospho-GSK 3b (Ser9) Kit

The AlphaScreen® SureFire® p-GSK 3β (Ser9) assay is a sandwich immunoassay for quantitative detection of phospho-GSK3β (phosphorylated on Ser9) in cellular lysates using Alpha Technology.
Price Range : 1750.00 CAD - 19100.00 CAD
SureFire principle

AlphaScreen SureFire mTOR (p-Ser2448) Assay Kit

The AlphaScreen® SureFire® p-mTOR (Ser2448) assay is a sandwich immunoassay for quantitative detection of phospho-mTOR (phosphorylated on Ser2448) in cellular lysates using Alpha Technology.
Price Range : 1750.00 CAD - 19100.00 CAD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-mTOR (Ser2448) Assay Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-mTOR (Ser2448) assay is a sandwich immunoassay for quantitative detection of phospho-mTOR (phosphorylated on Ser2448) in cellular lysates using Alpha no-wash technology.
List Price : 1950.00 CAD

ATP, [γ-32P]- 6000Ci/mmol 10mCi/ml

Adenosine triphosphate, labeled on the gamma phosphate group with 32P
Price Range : 630.00 CAD - 1350.00 CAD
PerkinElmer

LANCE Ultra Europium-anti-phospho-p70 S6K (Thr389) Antibody

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human p70 S6K phosphorylated at Thr389. p70 S6K is phosphorylated in vivo by mTOR. p70 S6K peptides are also phosphorylated by several kinases including COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1 and TAOK2. Sequence motif recognized: LGFTYVAP
Price Range : 1800.00 CAD - 8400.00 CAD
Alpha SureFire Ultra Multiplex kits

Alpha SureFire Ultra Multiplex p-AKT(Ser473) (Eu) + p-ERK (Tb) Kit

The Alpha SureFire® Ultra Multiplex kits allow the rapid, sensitive, and quantitative detection of phosphoproteins from cells, combined with the measurement of another protein, phospho or total. This Alpha Multiplex measurement is carried out in the same assay plate well from a single sample of cell lysate, and is achieved by the use of two types of Alpha Acceptor beads that emit at distinct wavelengths (545nm and 615nm).
Price Range : 1050.00 CAD - 60600.00 CAD
PerkinElmer

LANCE Ultra Europium anti-phospho-Glycogen Synthase (Ser641)

Europium-labeled rabbit polyclonal antibody recognizing human Muscle Glycogen Synthase phosphorylated at Ser641. Antibody labeled with the LANCE® Eu-W1024-ITC chelate. Sequence motif recognized: PApSVPPSPSL
Price Range : 2400.00 CAD - 8400.00 CAD

LANCE Ultra ULight™-poly GAT

Poly Glu-Ala-Tyr (1:1:1) labeled with ULight™ dye. This random polymer is a Tyrosine kinase substrate.
Price Range : 390.00 CAD - 1150.00 CAD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Ribosomal Protein S6 (Ser240/244) HV Assay Kit, 100 Assay Points

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-Ribosomal Protein S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha no-wash technology.
List Price : 580.00 CAD
SureFire principle

AlphaScreen SureFire EGFR (p-Tyr1068) Assay Kit

The AlphaScreen® SureFire® p-EGFR (Tyr1068) assay is a sandwich immunoassay for quantitative detection of phospho-EGFR (phosphorylated on Tyr1068) in cellular lysates using Alpha Technology.
Price Range : 1950.00 CAD - 21000.00 CAD

LANCE Ultra ULight-Glycogen Synthase (Ser641/pSer657) Peptide

Glycogen synthase peptide (Sequence: PASVPPSPSLSRHSSPHQ(pS)ED) labeled with ULight™ dye. Synthetic peptide containing residues surrounding Ser641 and Ser657 of human Muscle Glycogen Synthase. This peptide is pre-phosphorylated on Ser657. Target phosphorylation site: Ser641. This peptide substrate can be phosphorylated by a variety of kinases including GSK3α and GSK3β.
Price Range : 460.00 CAD - 1000.00 CAD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-eIF2α (Ser51) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-eIF2α (Ser51) assay is a sandwich immunoassay for quantitative detection of phospho-eIF2α (phosphorylated on Ser51) in cellular lysates using Alpha Technology.
Price Range : 580.00 CAD - 33600.00 CAD
PerkinElmer

ProfilerPro Kinase Peptide Substrate 16, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 16 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 720.00 CAD

GTP, [γ-32P]- 6000Ci/mmol 10mCi/ml EasyTide Lead, 250 µCi

Guanosine triphosphate (GTP), labeled on the gamma phosphate with 32P, is used as a substrate by some kinases in phosphorylation assays.
List Price : 740.00 CAD

LANCE Ultra Europium-anti-phospho-PLK (Ser137) antibody

Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from polo-like kinase (PLK) and phosphorylated at serine 137. PLK (Ser137) peptides can be phosphorylated by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Sequence motif recognized: RRRSLLE
Price Range : 1800.00 CAD - 8400.00 CAD
SureFire principle

AlphaScreen SureFire CREB (p-Ser133) Assay Kit

The AlphaScreen® SureFire® p-CREB (Ser133) assay is a sandwich immunoassay for quantitative detection of phospho-CREB (phosphorylated on Ser133) in cellular lysates using Alpha Technology.
Price Range : 1950.00 CAD - 20900.00 CAD
SureFire principle

AlphaScreen SureFire Phospho-Topo IIa (Ser1106) Assay Kit

The AlphaScreen® SureFire® p-Topoisomerase II alpha (p-Ser1106) assay is a sandwich immunoassay for quantitative detection of phospho-Topoisomerase II alpha (phosphorylated on Ser1106) in cellular lysates using Alpha Technology.
Price Range : 1750.00 CAD - 19100.00 CAD
SureFire principle

AlphaScreen SureFire MKK4 (p-Ser257;Thr261) Assay Kit

The AlphaScreen® SureFire® p-MKK4 (Ser257/Thr261) assay is a sandwich immunoassay for quantitative detection of phospho-MKK4 (phosphorylated on Ser257/Thr261) in cellular lysates using Alpha Technology.
Price Range : 1750.00 CAD - 19100.00 CAD

LANCE Ultra ULight™-Histone H3 (Thr3/Ser10) Peptide

ULight™-labeled synthetic peptide derived from residues 463-480 of human RAC-alpha serine/threonine-protein kinase (AKT1 or PKB). AKT/PKB can be phosphorylated at Ser473 by the mTOR/Rictor complex and MAPKAP kinase 2. Peptide sequence : CSERRPHFPQFSYSASGTAR
Price Range : 350.00 CAD - 1400.00 CAD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-IKKα (Ser176/180) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-IKKα (Ser176/180) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser176/180) in cellular lysates using Alpha Technology.
Price Range : 580.00 CAD - 33600.00 CAD
SureFire principle

AlphaScreen SureFire Phospho-cofilin (Ser3) Assay Kit

The AlphaScreen® SureFire® p-Cofilin (Ser3) assay is a sandwich immunoassay for quantitative detection of phospho-Cofilin (phosphorylated on Ser3) in cellular lysates using Alpha Technology.
Price Range : 1750.00 CAD - 19100.00 CAD

ATP, [γ-32P]- 3000Ci/mmol 10mCi/ml

Adenosine triphosphate, labeled on the gamma phosphate group with 32P
Price Range : 440.00 CAD - 1000.00 CAD

GTPγS, [35S]- 1250Ci/mmol, 12.5mCi/ml

Guanosine triphosphate, labeled on the gamma phosphate group with 35S (GTPγS).
Price Range : 1600.00 CAD - 2800.00 CAD
SureFire principle

AlphaScreen SureFire Phospho-PDK 1 (Ser241) Kit

The AlphaScreen® SureFire® p-PDK1 (p-Ser241) assay is a sandwich immunoassay for quantitative detection of phospho-PDK1 (phosphorylated on Ser241) in cellular lysates using Alpha Technology.
Price Range : 1750.00 CAD - 19100.00 CAD

"Kinase" 1-50 of 248 Products and Services

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