PerkinElmer

In Situ Hybridization

OF

Reliably detect and quantitate many nucleic acids that cannot be measured with standard methods using in situ hybridization. Our reagent kits are widely cited for detection of DNA, mRNA and miRNA sequences, with some researchers reporting detection of single copies.

Signal amplification with our kits leads to reliable results, even with short synthetic oligonucleotide probes. These can be used with any hapten labeled probe for fluorescent or chromogenic signal generation.

Advantages of using In Situ Hybridization:

  • Improved limit of detection and quantitative range
  • Reduced exposure time for faster imaging
  • Excellent localization of signal to the target

Tumor Microenvironment Opal 7 Color Image
Tumor Microenvironment Opal 7 Color Image

Stain

Simultaneously detect six biomarkers plus DAPI within a single tissue section. Similar to standard immunohistochemistry, our advanced method is accessible to many laboratories where IHC method development is performed. It allows researchers to use the best primary antibodies, even those raised in the same species, together for multiplex detection. Our staining solutions provide researchers with tools to assess multiple cellular phenotypes at the same time while retaining context provided within tissue sections.
Vectra® Polaris™ Automated Quantitative Pathology Imaging System
Vectra® Polaris™ Automated Quantitative Pathology Imaging System

Image

Our quantitative pathology imaging systems deliver high quality data where morphological context is preserved down to the sub-cellular level, removing autofluorescence and increasing signal-to-noise. These systems detect and measure multiple biomarkers, even if overlapping, within a single IHC or IF tissue section or TMA. Low signal levels that were previously undetected can now be captured, and overall quantification is vastly improved - particularly for solid, formalin-fixed, and paraffin-embedded tissues.
inform Image Analysis Software for Digital Pathology
inform Image Analysis Software for Digital Pathology

Analyze

Our software programs combine the latest technologies with algorithms and intuitive, easy-to-use interfaces, giving you the power to make new, exciting discoveries from your data. Our automated advanced image analysis software allows you to visualize, analyze, quantify and phenotype cells labeled with multiple biomarkers in situ in FFPE tissue sections.
Contract Research Services for Phenotyping In Situ
Contract Research Services for Phenotyping In Situ

Contract Research Services

Interested in testing the Phenoptics™ workflow? Our Research Services team can perform multiplexed IHC for up to seven colors (six markers plus DAPI) to help you get results.
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Application
  • Immunohistochemistry (IHC) (25)
  • In situ hybridization (ISH) (25)
  • Molecular Biology (14)
Buffer
  • Tricine (2)
  • Tricine with stabilizer and dye (12)
Concentration
  • 1 mm (1)
  • 10 mCi/mL (10)
  • 12.5 mCi/mL (1)
Detection Method
  • Chromogenic (6)
  • Fluorescence (69)
  • Radiometric (23)
Fluorophore
  • Coumarin (1)
  • Cy3 (19)
  • Cy3.5 (1)
  • Cy5 (16)
  • Cy5.5 (1)
  • Fluorescein (17)
  • Lissamine (1)
  • R110 (3)
  • R6G (3)
  • ROX (2)
  • TAMRA (4)
  • Texas Red (3)
  • TMR (5)
Format
  • Slides (25)
Label Position
  • alpha (11)
  • gamma (3)
Nucleotide
  • ATP (5)
  • CTP (5)
  • dATP (8)
  • dCTP (12)
  • ddATP (6)
  • ddCTP (6)
  • ddGTP (6)
  • ddUTP (7)
  • dGTP (4)
  • dTTP (1)
  • dUTP (9)
  • GTP (3)
  • UTP (11)
Product Brand Name
  • Ancillary Products (2)
  • EasyTide (7)
  • NEN (2)
  • NEN Radiochemicals (5)
  • Opal (1)
  • TSA (12)
  • TSA Plus (14)
Radionuclide
  • 33P (12)
  • 35S (2)
Radioisotope
  • P-33 (12)
  • S-35 (2)
Type
  • Opal 520 (1)
  • Opal 540 (1)
  • Opal 570 (1)
  • Opal 620 (1)
  • Opal 650 (1)
  • Opal 690 (1)
Volume Capacity
  • 150 µL (2)

"In Situ Hybridization" 1-50 of 115 Products and Services

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TSA Fluorescein System

TSA Fluorescein System

TSA® fluorescein detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra fluorescein in the localized area of the probe or antibody.
Price Range : 570.00 CAD - 1000.00 CAD
TSA Coumarin System (100-300 slides)

TSA Coumarin System, for 100-300 Slides

TSA® coumarin detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra coumarin in the localized area of the probe or antibody.
List Price : 1000.00 CAD
Amplification Diluent

TSA Tetramethylrhodamine Tyramide Reagent Pack, Reagents for 100-300 Slides

TSA® tetramethylrhodamine (TMR) reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra biotin in the localized area of the probe or antibody.
List Price : 800.00 CAD
PerkinElmer

Biotin-11-dATP 1mM 25nmol

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 390.00 CAD
Opal 4-Color Automation IHC Kit 50 slides - NEL800001KT

Opal Automation IHC Kit 50 slides

Opal is a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image. The method is similar to standard immunohistochemistry (IHC) and is accessible to many laboratories where standard IHC method development is performed.
Price Range : 1050.00 CAD - 1400.00 CAD

Fluorescein-12-ddCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 550.00 CAD

GTP, [α-33P]- 3000Ci/mmol, 10mCi/ml

Guanosine triphosphate, labeled on the alpha phosphate group with 33P.
Price Range : 560.00 CAD - 1600.00 CAD
PerkinElmer

Fluorescein-12-GTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 400.00 CAD
PerkinElmer

Cyanine 3-ddGTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 630.00 CAD

dATP, [α-33P]- 3000Ci/mmol, 10mCi/ml

Deoxyadenosine triphosphate, labeled on the alpha phosphate group with 33P.
Price Range : 630.00 CAD - 1600.00 CAD
TSA Tetramethylrhodamine System

TSA Tetramethylrhodamine System, for 100-300 Slides

TSA® tetramethylrhodamine (TMR) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra TMR in the localized area of the probe or antibody.
List Price : 1000.00 CAD
TSA Plus Cyanine 5/Fluorescein System

TSA Plus Cyanine 5 and Flourescein System

TSA® Plus multi-fluorophore detection kit, for amplification of multiple signals in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols.
List Price : 920.00 CAD
PerkinElmer

Biotin-11-dCTP 5mM 1,000nmol

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 12800.00 CAD
PerkinElmer

TSA Plus Biotin

TSA® Plus Biotin detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra biotin in the localized area of the probe or antibody.
Price Range : 500.00 CAD - 1850.00 CAD

UTP, [α-33P]- 3000Ci/mmol, 10mCi/ml

Uridine triphosphate, labeled on the alpha phosphate group with 33P.
Price Range : 1000.00 CAD - 1900.00 CAD
PerkinElmer

TSA Biotin Reagent Pack

TSA® biotin reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra biotin in the localized area of the probe or antibody.
Price Range : 1200.00 CAD - 5500.00 CAD
Opal 520 Reagent Pack

Opal Reagent Packs

Opal is a practical workflow for simultaneous detection of up to 6 tissue biomarkers plus nuclear counterstain, within a single image. The method is similar to standard immunohistochemistry and is accessible to many laboratories where standard IHC method development is performed.
Price Range : 145.00 CAD - 165.00 CAD
Tissue image

Opal Multiplex Manual IHC Kit 50 slides

Opal is a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image. The method is similar to standard immunohistochemistry (IHC) and is accessible to many laboratories where standard IHC method development is performed.
Price Range : 1150.00 CAD - 1450.00 CAD

dATP, [α-33P]- 3000Ci/mmol, 10mCi/ml

Deoxyadenosine triphosphate, labeled on the alpha phosphate group with 33P.
Price Range : 630.00 CAD - 1600.00 CAD

TSA Plus Cyanine 3.5

TSA® Plus Cyanine 3.5 (Cy3.5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy3.5 in the localized area of the probe or antibody.
Price Range : 290.00 CAD - 2350.00 CAD
PerkinElmer

R110-ddUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 540.00 CAD

Fluorescein-12-dUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 340.00 CAD
PerkinElmer

Cyanine 3-dUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 550.00 CAD

dTTP, [α-33P]- 3000Ci/mmol, 10mCi/ml, 1 mCi

Deoxythymidine triphosphate, labeled on the alpha phosphate group with 33P.
List Price : 1950.00 CAD
PerkinElmer

Cyanine 5-UTP, 100 nmol

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 600.00 CAD
PerkinElmer

3-Amino-3-Deoxydigoxigenin-9-dCTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 390.00 CAD
PerkinElmer

Fluorescein-12-dATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 390.00 CAD
PerkinElmer

R110-ddGTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 540.00 CAD
PerkinElmer

TSA Plus Cyanine 3

TSA® Plus Cyanine 3 (Cy3) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy3 in the localized area of the probe or antibody.
Price Range : 290.00 CAD - 2350.00 CAD
TSA -Plus Tetramethylrhodamine System

TSA Plus TMR

TSA® Plus tetramethylrhodamine (TMR) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra TMR in the localized area of the probe or antibody.
Price Range : 530.00 CAD - 2350.00 CAD
PerkinElmer

TAMRA-ddCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 540.00 CAD

CTP, [α-33P]- 3000Ci/mmol, 10mCi/ml

Cytidine triphosphate, labeled on the alpha phosphate group with 33P.
Price Range : 530.00 CAD - 1750.00 CAD
PerkinElmer

Cyanine 3-dCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 550.00 CAD

ATP, [γ-33P]- 3000Ci/mmol, 10mCi/ml

Adenosine triphosphate, labeled on the gamma phosphate group with 33P.
Price Range : 510.00 CAD - 1400.00 CAD
PerkinElmer

Biotin-16 UTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 390.00 CAD
PerkinElmer

TSA Plus Cyanine 5.5

TSA® Plus Cyanine 5.5 (Cy5.5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy5.5 in the localized area of the probe or antibody.
Price Range : 290.00 CAD - 2350.00 CAD
PerkinElmer

Biotin-11-dATP 5mM 75nmol

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 1050.00 CAD
PerkinElmer

TSA Plus Cyanine 3 System, for 50-150 Slides

TSA® Plus Cyanine 3 (Cy3) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy3 in the localized area of the probe or antibody.
List Price : 530.00 CAD

R6G-ddATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 540.00 CAD

Cyanine 5-dCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 550.00 CAD

Fluorescein-12-dCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 390.00 CAD

Fluorescein-12-ATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 390.00 CAD

UTPαS, [35S]- 800Ci/mmol, 40mCi/ml, 1 mCi

Uridine triphosphate, labeled on the alpha phosphate group with 35S.
List Price : 2050.00 CAD
PerkinElmer

Cyanine 5-CTP, 100 nmol

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 590.00 CAD
PerkinElmer

Cyanine Smart Pack dCTP

Each Cyanine Smart Pack dCTP contains 5 tubes each of cyanine 3 and cyanine 5 labeled deoxycytidine triphosphate (dCTP). Smart packs are ideal for microarray labeling and other molecular biology applications.
List Price : 4450.00 CAD
PerkinElmer

Cyanine Smart Pack UTP

Each Cyanine Smart Pack UTP contains 5 tubes each of cyanine 3 and cyanine 5 labeled uridine triphosphate (UTP). Smart packs are ideal for microarray labeling and other molecular biology applications.
List Price : 4750.00 CAD
PerkinElmer

Cyanine 5-ddUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 630.00 CAD
PerkinElmer

TSA Plus Fluorescein

TSA® Plus fluorescein detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra fluorescein in the localized area of the probe or antibody.
Price Range : 290.00 CAD - 2350.00 CAD

ATP, [γ-33P]- 3000Ci/mmol, 10mCi/ml

Adenosine triphosphate, labeled on the gamma phosphate group with 33P.
Price Range : 850.00 CAD - 2150.00 CAD
PerkinElmer

3-Amino-3-Deoxydigoxigenin-9-ddGTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 540.00 CAD

"In Situ Hybridization" 1-50 of 115 Products and Services

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