PerkinElmer

In Situ Hybridization

OF

Reliably detect and quantitate many nucleic acids that cannot be measured with standard methods using in situ hybridization. Our reagent kits are widely cited for detection of DNA, mRNA and miRNA sequences, with some researchers reporting detection of single copies.

Signal amplification with our kits leads to reliable results, even with short synthetic oligonucleotide probes. These can be used with any hapten labeled probe for fluorescent or chromogenic signal generation.

Advantages of using In Situ Hybridization:

  • Improved limit of detection and quantitative range
  • Reduced exposure time for faster imaging
  • Excellent localization of signal to the target

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Application
  • Immunohistochemistry (IHC) (25)
  • In situ hybridization (ISH) (25)
  • Molecular Biology (14)
Buffer
  • Tricine (2)
  • Tricine with stabilizer and dye (12)
Concentration
  • 1 mm (1)
  • 10 mCi/mL (10)
  • 12.5 mCi/mL (1)
Detection Method
  • Chromogenic (6)
  • Fluorescence (69)
  • Radiometric (23)
Fluorophore
  • Coumarin (1)
  • Cy3 (19)
  • Cy3.5 (1)
  • Cy5 (16)
  • Cy5.5 (1)
  • Fluorescein (17)
  • Lissamine (1)
  • R110 (3)
  • R6G (3)
  • ROX (2)
  • TAMRA (4)
  • Texas Red (3)
  • TMR (5)
Format
  • Slides (25)
Label Position
  • alpha (11)
  • gamma (3)
Nucleotide
  • ATP (5)
  • CTP (5)
  • dATP (8)
  • dCTP (12)
  • ddATP (6)
  • ddCTP (6)
  • ddGTP (6)
  • ddUTP (7)
  • dGTP (4)
  • dTTP (1)
  • dUTP (9)
  • GTP (3)
  • UTP (11)
Product Brand Name
  • Ancillary Products (2)
  • EasyTide (7)
  • NEN (2)
  • NEN Radiochemicals (5)
  • Opal (1)
  • TSA (12)
  • TSA Plus (14)
Radionuclide
  • 33P (12)
  • 35S (2)
Radioisotope
  • P-33 (12)
  • S-35 (2)
Type
  • Opal 520 (1)
  • Opal 540 (1)
  • Opal 570 (1)
  • Opal 620 (1)
  • Opal 650 (1)
  • Opal 690 (1)
Volume
  • 0-50 (14)
  • 51-100 (8)
  • 201-250 (11)
Volume Capacity
  • 150 µL (2)
  • 25 nmol (2)
  • 75 nmol (1)

"In Situ Hybridization" 1-50 of 115 Products and Services

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Amplification Diluent

TSA Fluorescein Reagent Pack

TSA® fluorescein reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra biotin in the localized area of the probe or antibody.
Price Range : 800.00 CAD - 3750.00 CAD
TSA Plus DNP (AP)System

TSA Plus DNP (AP)

TSA® Plus DNP (AP) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. The deposited DNP is detected using an anti-DNP alkaline phosphatase, with appropriate substrate.
Price Range : 340.00 CAD - 600.00 CAD
PerkinElmer

Anti-DNP HRP Conjugate

Anti-DNP antibody conjugated to horseradish peroxidase
List Price : 340.00 CAD

dCTP, [α-33P]- 3000Ci/mmol, 10mCi/ml

Deoxycytidine triphosphate, labeled on the alpha phosphate group with 33P.
Price Range : 610.00 CAD - 1950.00 CAD

Fluorescein-12-UTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 400.00 CAD
PerkinElmer

Cyanine Smart Pack UTP

Each Cyanine Smart Pack UTP contains 5 tubes each of cyanine 3 and cyanine 5 labeled uridine triphosphate (UTP). Smart packs are ideal for microarray labeling and other molecular biology applications.
List Price : 4750.00 CAD
PerkinElmer

TSA Plus DIG Evaluation Kit

TSA® Plus DIG detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra DIG in the localized area of the probe or antibody.
Price Range : 280.00 CAD - 1850.00 CAD

Fluorescein-12-dCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 390.00 CAD

GTP, [α-33P]- 3000Ci/mmol, 10mCi/ml

Guanosine triphosphate, labeled on the alpha phosphate group with 33P.
Price Range : 560.00 CAD - 1600.00 CAD

R6G-ddCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 540.00 CAD
Opal 650 Reagent Pack

Opal Reagent Packs

Opal is a practical workflow for simultaneous detection of up to 6 tissue biomarkers plus nuclear counterstain, within a single image. The method is similar to standard immunohistochemistry and is accessible to many laboratories where standard IHC method development is performed.
Price Range : 145.00 CAD - 165.00 CAD
PerkinElmer

TSA Plus Cyanine 5

TSA® Plus Cyanine 5 (Cy5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy5 in the localized area of the probe or antibody.
Price Range : 290.00 CAD - 2350.00 CAD
PerkinElmer

TSA Plus DNP (HRP)

TSA® Plus DNP (HRP) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. The deposited DNP is detected using an anti-DNP hroseradish peroxidase, with appropriate substrate.
Price Range : 340.00 CAD - 600.00 CAD
PerkinElmer

TSA Cyanine 5 System, for 50-150 Slides

TSA® Cyanine 5 (Cy5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy5 in the localized area of the probe or antibody.
List Price : 570.00 CAD
PerkinElmer

Fluorescein-12-GTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 400.00 CAD
PerkinElmer

Cyanine Smart Pack dUTP

Each Cyanine Smart Pack dCTP contains 5 tubes each of cyanine 3 and cyanine 5 labeled deoxycytidine triphosphate (dCTP). Smart packs are ideal for microarray labeling and other molecular biology applications.
List Price : 4450.00 CAD
PerkinElmer

Texas Red®-5-dATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 400.00 CAD
PerkinElmer

Fluorescein-12-dATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 390.00 CAD
PerkinElmer

Cyanine 3-dATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 550.00 CAD
PerkinElmer

Cyanine 5-ddUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 630.00 CAD

ATP, [γ-33P]- 3000Ci/mmol, 10mCi/ml

Adenosine triphosphate, labeled on the gamma phosphate group with 33P.
Price Range : 850.00 CAD - 1350.00 CAD
PerkinElmer

TSA Fluorescein System

TSA® fluorescein detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra fluorescein in the localized area of the probe or antibody.
Price Range : 570.00 CAD - 1000.00 CAD

Texas Red-5-dUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 390.00 CAD
PerkinElmer

Fluorescein-12-ddUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 550.00 CAD

TSA Plus Cyanine 5.5

TSA® Plus Cyanine 5.5 (Cy5.5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy5.5 in the localized area of the probe or antibody.
Price Range : 290.00 CAD - 2350.00 CAD
PerkinElmer

Biotin-11-GTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 390.00 CAD
PerkinElmer

Cyanine 5-dATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 550.00 CAD
PerkinElmer

Anti-DNP AP Conjugate

Anti-DNP antibody conjugated to alkaline phosphatase
List Price : 400.00 CAD
Amplification Diluent

TSA Cyanine 5 Tyramide Reagent Pack, Reagents for 50-150 Slides

TSA® Cyanine 5 (Cy5) reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra biotin in the localized area of the probe or antibody.
List Price : 460.00 CAD

dATP, [α-33P]- 3000Ci/mmol, 10mCi/ml

Deoxyadenosine triphosphate, labeled on the alpha phosphate group with 33P.
Price Range : 630.00 CAD - 1600.00 CAD

TSA Plus Cyanine 3.5

TSA® Plus Cyanine 3.5 (Cy3.5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy3.5 in the localized area of the probe or antibody.
Price Range : 290.00 CAD - 2350.00 CAD
PerkinElmer

Cyanine 5-UTP, 100 nmol

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 600.00 CAD
PerkinElmer

TSA Plus Fluorescein

TSA® Plus fluorescein detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra fluorescein in the localized area of the probe or antibody.
Price Range : 290.00 CAD - 2350.00 CAD
PerkinElmer

Cyanine 5-ddCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 630.00 CAD
PerkinElmer

R110-ddUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 540.00 CAD

Fluorescein-12-CTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 400.00 CAD
PerkinElmer

DNP-11-UTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 390.00 CAD

dATP, [α-33P]- 3000Ci/mmol, 10mCi/ml

Deoxyadenosine triphosphate, labeled on the alpha phosphate group with 33P.
Price Range : 630.00 CAD - 1600.00 CAD
PerkinElmer

3-Amino-3-Deoxydigoxigenin-9-dCTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 390.00 CAD
PerkinElmer

Cyanine 3-dGTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 550.00 CAD

UTP, [α-33P]- 3000Ci/mmol, 10mCi/ml

Uridine triphosphate, labeled on the alpha phosphate group with 33P.
Price Range : 560.00 CAD - 1750.00 CAD
PerkinElmer

DNP-11-dUTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 390.00 CAD

Texas Red®-5-dCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 390.00 CAD
PerkinElmer

Biotin-11-dUTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 390.00 CAD
PerkinElmer

TSA Plus Biotin

TSA® Plus Biotin detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra biotin in the localized area of the probe or antibody.
Price Range : 500.00 CAD - 1850.00 CAD

CTP, [α-33P]- 3000Ci/mmol, 10mCi/ml

Cytidine triphosphate, labeled on the alpha phosphate group with 33P.
Price Range : 530.00 CAD - 1750.00 CAD
PerkinElmer

TSA Biotin System

TSA® biotin detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra biotin in the localized area of the probe or antibody.
Price Range : 440.00 CAD - 1500.00 CAD
PerkinElmer

Biotin-11-ddUTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
List Price : 540.00 CAD
TSA Plus Cyanine 5/Fluorescein System

TSA Plus Cyanine 5 and Flourescein System

TSA® Plus multi-fluorophore detection kit, for amplification of multiple signals in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols.
List Price : 920.00 CAD
PerkinElmer

Cyanine 3-ddGTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
List Price : 630.00 CAD

"In Situ Hybridization" 1-50 of 115 Products and Services

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