PerkinElmer

Drug Discovery Reagents

OF

Drug discovery is a complex, costly, and time-consuming undertaking. And for research scientists, high-throughput screening is where the journey to discovery begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our HTS screening reagents do just that, providing you with a wide range of validated reagents and kits in Alpha, LANCE®, DELFIA®, and other assay technologies.

Accelerate your workflow, while getting consistent, accurate, and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

 

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein:protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE® Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

 

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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AlphaLisa Analyte 2

IgA (human), lyophilized (AlphaLISA)

Human IgA standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human IgA kit, but can be ordered separately.
List Price : 290.00 CAD

HGFR/c-MET (human) AlphaLISA Detection Kit

The AlphaLISA® Human HGFR / c-MET Detection Kit is designed for detection and quantitation of HGFR / c-MET in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The antibodies target the extracellular domain of HGFR. The analyte in this kit consists of the extracellular domain of human HGFR fused to the Fc region of human IgG1.
Price Range : 3450.00 CAD - 27000.00 CAD
AlphaLisa Analyte 2

IGF2 (human), lyophilized (AlphaLISA)

Human Insulin-like Growth Factor 2 (IGF2) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human IGF2 kit, but can be ordered separately.
List Price : 290.00 CAD

Glutathione AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to glutathione. These beads can be used to capture GST-tagged proteins and peptides.
Price Range : 700.00 CAD - 28600.00 CAD
AlphaLISA immunoassay kits

IgG (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for detection and quantitation of human IgG in buffer or cell culture supernatant allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development. The antibodies in the kit target the Fcγ region.
Price Range : 900.00 CAD - 7500.00 CAD

IL-12/IL-23 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-12 enables the detection and quantitation of human Interleukin 12 p40 (IL12 p40) in human serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). It is important to note that this assay will also detect IL-23 p40.
Price Range : 820.00 CAD - 23200.00 CAD
AlphaLisa Analyte 2

GAD67 (Human) lyophilized (AlphaLISA)

Human GAD67 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA GAD67 Detection Kit, but can be ordered separately.
List Price : 350.00 CAD
PerkinElmer

IFNα (human), lyophilized (LANCE)

Human IFNα standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human IFNα Detection Kit, but can also be ordered separately.
List Price : 290.00 CAD
AlphaLISA immunoassay kits

IFNγ (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA Human IFNγ Biotin-Free Detection Kit is designed for the quantitative detection of human IFNγ in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 820.00 CAD - 11900.00 CAD

TSH (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human TSH Detection Kit is designed for detection and quantitation of human thyroid stimulating hormone in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 940.00 CAD - 12600.00 CAD

DELFIA Sm-Labeling kit (Sm-N1-ITC Chelate), 0.2mg

The DELFIA® Sm-Labeling kit is intended for labeling of proteins with samarium (Sm), for use in multiplexing dissociation enhanced time resolved fluoroimmunoassays.
List Price : 640.00 CAD
AlphaLISA immunoassay kits

CXCL12/SDF-1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CXCL12 (SDF-1) enables the quantitative determination of human CXCL12 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 510.00 CAD - 11200.00 CAD

IgG (mouse) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse IgG Detection Kit is designed for detection and quantitation of Mouse IgG in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 940.00 CAD - 12600.00 CAD

Nickel Chelate AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to nickel chelate. These beads can be used to capture His-tagged proteins and peptides.
Price Range : 700.00 CAD - 28600.00 CAD
PerkinElmer

IFNα (mouse), lyophilized (LANCE)

Mouse IFNα standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Mouse IFNα Detection Kit, but can also be ordered separately.
List Price : 290.00 CAD
AlphaLISA immunoassay kits

IL-17A (human) AlphaLISA Biotin-Free Detection Kit

AlphaLISA Human IL-17A Biotin-Free Detection Kit is designed for the quantitative detection of human IL-17A in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin.
Price Range : 820.00 CAD - 11900.00 CAD

Anti-mouse IgG (Fc specific) AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to anti-mouse IgG antibody. These beads can be used to capture mouse IgG antibodies. The antibody is Fc-specific, and is a goat polyclonal F(ab')2 fragment.
Price Range : 700.00 CAD - 28600.00 CAD
AlphaLISA immunoassay kits

IL-15 (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA® Human IL-15 Biotin-Free Detection Kit is designed for the quantitative detection of human Interleukin 15 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.
Price Range : 530.00 CAD - 11500.00 CAD
LANCE_Fc_receptor

FCGR3A/CD16A (176Phe/F158) (human) LANCE Ultra TR-FRET Binding Kit

This kit is designed for the quantitative determination of Human FCGR3A/CD16a (176Phe/F158) and human Fc fragment binding using a homogeneous (no wash) LANCE Ultra TR-FRET binding assay.
Price Range : 920.00 CAD - 12200.00 CAD
AlphaLISA immunoassay kits

Alpha-synuclein (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human Alpha-synuclein enables the quantitative determination of Alpha-synuclein in biological fluids such as serum, plasma, cerebrospinal fluid and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 760.00 CAD - 12000.00 CAD
AlphaPlex Samarium Acceptor Beads

Unconjugated AlphaPlex™ 545 Acceptor Beads

Unconjugated AlphaPlex 645 (Samarium) Acceptor beads bear reactive aldehyde groups at their surface, allowing you to conjugate a wide variety of reagents using a simple reductive amination protocol.
Price Range : 1600.00 CAD - 33500.00 CAD

Cry2A AlphaLISA Detection Kit

The AlphaLISA® Cry2A Detection Kit is designed for detection and quantitation of Cry2A in GMO seeds, leaves, and plant extracts in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 710.00 CAD - 11000.00 CAD
Protein

IgG (mouse), lyophilized (LANCE)

Mouse IgG standard, lyophilized, for use in running standard curves in LANCE TR-FRET no-wash detection assays. This standard is already provided in the LANCE Ultra Mouse IgG TR-FRET Detection Kit, but can be ordered separately.
List Price : 280.00 CAD
AlphaLISA immunoassay kits

EGFR (human) AlphaLISA Detection Kit

The AlphaLISA® EGFR Detection Kit is designed for detection and quantitation of human Epidermal Growth Factor Receptor (EGFR) in cell culture media, human serum, and other sample types using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 820.00 CAD - 11900.00 CAD
AlphaLISA assay principle

IL-31 (Human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for IL-31 enables the quantitative determination of human IL-31 in serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with mouse IL31. Cross-reactivity with other species has not been tested.
Price Range : 730.00 CAD - 22000.00 CAD

IFN-α (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IFN-α Detection Kit is designed for detection and quantitation of human interferon α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 940.00 CAD - 12500.00 CAD
AlphaLisa Analyte 2

IgM (human), lyophilized (AlphaLISA)

Human IgM standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human IgM kit, but can be ordered separately.
List Price : 290.00 CAD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total ERK 1/2 Assay Kit

The AlphaLISA® SureFire® Ultra™ Total ERK 1/2 assay is a sandwich immunoassay for quantitative detection of ERK 1/2 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 580.00 CAD - 33600.00 CAD

AlphaLISA Anti-O-linked GlcNAc Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against O-linked n-acetylglucosamine (O-linked GlcNAc). These beads can be used for AlphaLISA no-wash assays.
Price Range : 1700.00 CAD - 30600.00 CAD
AlphaLisa Analyte 2

IL-12 (p70) (human), lyophilized (AlphaLISA)

Human Interleukin 12 (p70) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human IL12 kit, but can be ordered separately.
List Price : 290.00 CAD
LANCE_Fc_receptor

FCGR3A/CD16a (176Val/V158) LANCE Ultra TR-FRET Binding Kit

This kit is designed for the quantitative determination of Human FCGR3A/CD16a (176Val/V158) and human Fc fragment binding using a homogeneous (no wash) LANCE Ultra TR-FRET binding assay.
Price Range : 920.00 CAD - 12200.00 CAD

DELFIA®; Eu-N1 Goat anti-Rabbit IgG

Europium-labeled anti-rabbit IgG antibody, for use in DELFIA® TRF assays
Price Range : 860.00 CAD - 3900.00 CAD
AlphaLisa Analyte 2

SCF (human), lyophilized (AlphaLISA)

Human stem cell factor (SCF; KIT-ligand) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human SCF Detection Kit, but can also be ordered separately.
List Price : 280.00 CAD

DELFIA Eu-N1 Anti-Phosphotyrosine (PY20)

Europium-labeled anti-phosphotyrosine (PY20) antibody, for use in DELFIA time-resolved fluorescence (TRF) kinase assays, phosphoprotein detection, and phosphoprotein binding assays.
Price Range : 310.00 CAD - 3850.00 CAD

IL-10 (bovine/ovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for bovine IL-10 enables the detection and quantitation of bovine IL-10 (bIL10) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). This kit also detects Ovine IL-10.
Price Range : 820.00 CAD - 14900.00 CAD
AlphaLISA Cellular Epigenetics kits

AlphaLISA Acetylated-Histone H3 Lysine 27 (H3K27ac) Cellular Detection Kit

This kit is designed for the detection of acetylated Histone H3 Lysine 27 (H3K27ac) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 820.00 CAD - 14700.00 CAD
AlphaPlex-545-unconjugated

Unconjugated AlphaPlex-545 Acceptor Beads

Unconjugated AlphaPlex 545 (Terbium) Acceptor beads bear reactive aldehyde groups at their surface, allowing you to conjugate a wide variety of reagents using a simple reductive amination protocol.
Price Range : 1600.00 CAD - 33500.00 CAD

IFN-γ (mouse) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse IFN-γ Detection Kit is designed for detection and quantitation of mouse interferon γ in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 940.00 CAD - 12500.00 CAD
AlphaLisa Analyte 2

p62 (human), lyophilized (AlphaLISA)

Human p-62 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA p-62 Detection Kit, but can be ordered separately.
List Price : 370.00 CAD
AlphaLISA immunoassay kits

Glucagon (human) AlphaLISA Detection Kit

The AlphaLISA® Glucagon Detection Kit is designed for detection and quantitation of glucagon in cell culture media, sera and plasma, in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 820.00 CAD - 11400.00 CAD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-ERK1/2 (Thr202/Tyr204) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-ERK 1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK 1/2 (phosphorylated on Thr202/Tyr204 in ERK1, or Thr185/Tyr187 in ERK2) in cellular lysates using Alpha Technology.
Price Range : 580.00 CAD - 33600.00 CAD
Protein

COX-2 (human), liquid (LANCE)

Human COX-2 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human COX-2 TR-FRET Detection Kit, but can also be ordered separately.
List Price : 350.00 CAD
AlphaLisa Analyte 2

IL-17F (human) Iyophilized (AlphaLISA)

Human IL17F standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA IL17F Detection Kit, but can be ordered separately.
List Price : 360.00 CAD

AlphaScreen DIG (Digoxin/Digoxigenin) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-Dig (digoxin) conjugated AlphaScreen Acceptor beads. 
Price Range : 215.00 CAD - 16300.00 CAD

DELFIA Eu-Labeling kit (Eu-N1-ITC chelate), 0.2mg

DELFIA® Europium labeling kit for labeling your own biomolecules for use in DELFIA time-resolved fluorescence assays. The ITC chelate reacts with free amine groups in the target biomolecule.
List Price : 640.00 CAD
neolite Reporter Gene Assay kit

neolite Reporter Gene Assay System

Glow luminescence reporter gene assay combining benefits of both bright signal and long-lasting signal for firefly luciferase.
Price Range : 100.00 CAD - 5800.00 CAD
AlphaLISA immunoassay kits

SCF (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human SCF enables the quantitative determination of stem cell factor (SCF; KIT-ligand) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 510.00 CAD - 11200.00 CAD

DELFIA Eu-N1 Anti-Phosphtyrosine (PT66)

Europium labeled anti-phosphotyrosine PT66 antibody for DELFIA® time-resolved fluorescence (TRF) kinase assays. The PT66 antibody is an affinity purified mouse monoclonal antibody that binds to phosphorylated tyrosine residues.
Price Range : 260.00 CAD - 3850.00 CAD
AlphaLISA immunoassay kits

TNFα (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine TNFα in serum, plasma, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 760.00 CAD - 12200.00 CAD
AlphaLISA immunoassay kits

PD-1/PD-L1 (human) AlphaLISA Binding Kit

The AlphaLISA® PD-1/PD-L1 Binding Kit is designed for the detection of binding activity between Human PD-1 and PD-L1, using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of therapeutics which competitively inhibit PD-1/PD-L1 interaction.
Price Range : 820.00 CAD - 11500.00 CAD

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