From receptor ligand binding to intracellular pathway analysis of phosphorylated proteins, breakthrough discoveries in cell signaling and signal transduction are coming from laboratories that maximize high-quality results while minimizing hassles.
We enable a deeper understanding and more biologically relevant results with complete cell-based and biochemical assay solutions featuring innovative reagent technologies like: AlphaLISA SureFire Ultra, AlphaLISA SureFire Ultra Multiplex, AlphaScreen SureFire, LANCE Ultra TR-FRET, and DELFIA TRF assays – not to mention our complete line of industry leading radiochemicals.
With a range of highly reliable, high-performance multimode plate readers, including the EnVision and EnSight systems, we provide access to a wide range of detection technologies for everything you need to bring about the next big breakthrough.
Alpha SureFire® assays are no-wash cellular kinase assays. While they exist in different formats (AlphaScreen® SureFire, AlphaLISA® SureFire Ultra, and Alpha SureFire Ultra Multiplex), all use PerkinElmer’s exclusive bead-based technology in a sandwich immunoassay format for the detection of phosphorylated proteins in cells. Alpha SureFire assays are a quantitative alternative to Western Blotting, are automation-friendly, miniaturizable, and detect both endogenous and recombinant proteins.
AlphaScreen SureFire has a rich track record of small molecule screening successes:
AlphaLISA SureFire Ultra
The labeling of one of the antibodies with CaptSure™, a small peptide tag, is recognized by CaptSure-antibody-coated beads, providing significant improvement over the AlphaScreen SureFire format:
Alpha SureFire Ultra Multiplex
This format utilizes AlphaPlex™ technology to read multiple signals from the same well:
We offer the widest selection of proven GPCR target solutions - beyond the ligand GPCR event to the actual assessment of the cellular-activated signal transduction cascade. Enabling you to identify, validate, screen, and profile your leads is our priority.
LANCE Ultra TR-FRET
LANCE® Ultra TR-FRET Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.
LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer) based, homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.
DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.
DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match.
Radiometric detection is a direct method that removes the need for target specific antibodies, reducing assay time & variables. A range of isotopes (3H, 14C, 33P, 35S) can be labeled to a variety of without altering biological function to study in the most natural state receptor ligand binding, kinases, G proteins, and other cell signaling pathways.
For high throughput screening, SPA (Scintillation Proximity Assay) technology provides a homogenous radiometric format for a multitude of assays.
An alternative format, SPA Imaging Beads are designed for detection on CCD based microplate imagers They offer additional benefits to SPA Scintillation Beads, including:
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The EnSight™ multimode plate reader combines labeled, label-free* & image-based cytometry technologies in a single benchtop platform. Imaging & online data analysis is fast: a 384-well plate can be imaged in less than 5 mins. The system’s modular design, combined with workflow-based Kaleido™ data acquisition & analysis software, gives a truly versatile plate reader that gets users productive quickly.
*Label free detection module of EnSight Plate Reader is available outside the EU. Please contact your local representative for further details.